Publications:50
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Achla Gupta, PhD
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About Me
Dr. Gupta is a member of the Devi Laboratory within the Department of Pharmacology and Systems Therapeutics.
Language
Position
About Me
Dr. Gupta is a member of the Devi Laboratory within the Department of Pharmacology and Systems Therapeutics.
Language
Position
Education
MPhil, Aligarh Muslim University
PhD, Aligarh Muslim University
Research
My research interests include the investigation of G-protein coupled
receptor heterodimerization/oligomerization using antibodies as tools. I have
generated an antibody against mu-delta heterodimers using subtractive
immunization. This antibody recognize mu-delta heterdimers by ELISA in vitro
and as well as in vivo. I have also generated selective monoclonal antibodies
against the N-terminal region of opioid receptors. We have used N-terminal
selective antibodies to investigate the structural changes occurring during
opioid receptor activation. Interestingly, antibody recognition was found to be
modulated only by agonists and not antagonists in a dose-dependent manner. The
antibodies were able to distinguish agonist-mediated conformational changes
between wild-type and constitutively active mutant receptors. Based on these
results, I have generated conformation-sensitive polyclonal antibodies against
a similar region in the N-terminus of other members of the rhodopsin family
(cannabinoid and adrenergic receptors). Interestingly, these antibodies were
also able to recognize active conformations of the endogenous receptors. These
antibodies provide a potential tool for identifying and characterizing new
compounds of therapeutic interest.
Locations
Publications
Selected Publications
- Multiomics Analyses Identify Proline Endopeptidase-Like Protein As a Key Regulator of Protein Trafficking, a Pathway Underlying Alzheimer's Disease Pathogenesis. Mariana Lemos Duarte, Minghui Wang, Ivone Gomes, Chenge Liu, Ali Sharma, Amanda K. Fakira, Achla Gupta, Seshat M. MacK, Bin Zhang, Lakshmi A. Devi. Molecular Pharmacology
- Interactions between cannabinoid and opioid receptors in a mouse model of diabetic neuropathy. Elaine F. Toniolo, Achla Gupta, Adriano C. Franciosi, Ivone Gomes, Lakshmi A. Devi, Camila S. Dale. Pain
- High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics. Mariana Lemos Duarte, Nikita A. Trimbake, Achla Gupta, Christine Tumanut, Xiaomin Fan, Catherine Woods, Akila Ram, Ivone Gomes, Erin N. Bobeck, Deborah Schechtman, Lakshmi A. Devi. Communications Biology